Conferencia: SUMOilación en plantas

El próximo viernes 9 de abril, a las 12 h, tendremos el placer de contar con la participación de la Dra. L María Lois, del Centro de Investigación en Genómica de la Agricultura (CRAG, Barcelona), que nos impartirá la conferencia titulada: SUMO conjugation in plants: mechanistic insight and biological implications. La Dr. Lois ha sido invitada por el profesor José Lozano (Dpto. Biología Molecular y Bioquímica UMA). Debido a la situación de emergencia sanitaria por COVID-19, la conferencia tendrá lugar online a través de la plataforma Google Meet. Podréis acceder mediante el siguiente enlace: meet.google.com/ncg-ueva-pyt

Publicaciones representativas:

Benlloch, R., Maria Lois, L.
Sumoylation in plants: Mechanistic insights and its role in drought stress
(2018) Journal of Experimental Botany, 69(19): 4539-4554

Castaño-Miquel, L., Mas, A., Teixeira, I., Seguí, J., Perearnau, A., Thampi, B.N., Schapire, A.L., Rodrigo, N., La Verde, G., Manrique, S., Coca, M., Lois, L.M.
SUMOylation Inhibition Mediated by Disruption of SUMO E1-E2 Interactions Confers Plant Susceptibility to Necrotrophic Fungal Pathogens
(2017) Molecular Plant, 10(5): 709-720

Liu, B., Lois, L.M., Reverter, D.
Structural analysis and evolution of specificity of the SUMO UFD E1-E2 interactions
(2017) Scientific Reports, 7: 41998

Castaño-Miquel, L., Maria Lois, L.
Kinetic analysis of plant SUMO conjugation machinery
(2016) . In: Lois LM, Matthiesen R, eds. Plant Proteostasis: Methods and Protocols. Methods in Molecular Biology, 1450:107-123

Schapire, A.L., Maria Lois, L.
A simplified and rapid method for the isolation and transfection of Arabidopsis leaf mesophyll protoplasts for large-scale applications
(2016) In: Botella JR, Botella MA, eds. Plant Signal Transduction.  Humana Press. Methods in Molecular Biology, 1363:79-88

Castaño-Miquel, L., Segui, J., Manrique, S., Teixeira, I., Carretero-Paulet, L., Atencio, F., Lois, L.M.
Diversification of SUMO-activating enzyme in arabidopsis: Implications in SUMO conjugation
(2013) Molecular Plant, 6(5): 1646-1660

Castaño-Miquel, L., Seguí, J., Lois, L.M.
Distinctive properties of Arabidopsis SUMO paralogues support the in vivo predominant role of AtSUMO1/2 isoforms
(2011) Biochemical Journal, 436(3): 581-590

Chosed, R., Mukherjee, S., Lois, L.M., Orth, K.
Evolution of a signalling system that incorporates both redundancy and diversity: Arabidopsis SUMOylation
(2006) Biochemical Journal, 398(3):521-529

Lois, L.M., Lima, C.D.
Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1
(2005) EMBO Journal, 24(3): 439-451

Conferencia: Alzheimer y nanomedicina.

El próximo viernes 19 de marzo, a las 12 h, tendrá lugar la 4ª conferencia de nuestro ciclo 2020-21. En esta ocasión, la Dra. Joana A. Loureiro (LEPABE, Oporto, Portugal) nos impartirá la conferencia Liposomebased nanocarriers to transport drugs for the Alzheimer´s disease treatment. La Dr. Loureiro ha sido invitada por la investigadora Inés Moreno (Dpto. Biología Celular, Genética y Fisiología, UMA). Debido a la situación de emergencia sanitaria por COVID-19, la conferencia tendrá lugar online a través de la plataforma Google Meet. Podréis acceder mediante el siguiente enlace: meet.google.com/ccc-ntvn-sei

Breve reseña de la trayectoria investigadora: Dr. Joana A. Loureiro is a researcher at the Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE) since 2010 and Invited Assistant Professor at Faculty of Engineering, University of Porto (FEUP) since 2018. Joana did her bachelors and masters in chemical engineering followed by a bachelors and masters in pharmaceutical sciences. She received her PhD degree in chemical and biological engineering from FEUP in 2013. Since 2010, Joana has been working in the field of drug delivery nanosystems for brain diseases treatment. She has expertise in protein misfolding and pathogenic biomarkers associated with Alzheimer’s disease. Her main research areas of interest comprises: i) nanotechnology and interfacial phenomena; ii) effects of fluorinated systems and peptides on the aggregation of amyloid-beta peptide; iii) conformational studies of proteins and peptides self-organized systems and polymer surfaces; and iv) design and production of inorganic and polymeric nano-systems for pharmaceutical application.

Recent publications:

In vivo Bio-Distribution and Toxicity Evaluation of Polymeric and Lipid-Based Nanoparticles: A Potential Approach for Chronic Diseases Treatment. Fonseca-Gomes J, Loureiro JA, Tanqueiro SR, Mouro FM, Ruivo P, Carvalho T, Sebastião AM, Diógenes MJ, Pereira MC. Int J Nanomedicine. 2020 Nov 5;15:8609-8621.

PLGA Based Drug Carrier and Pharmaceutical Applications: The Most Recent Advances. Loureiro JA, Pereira MC. Pharmaceutics. 2020 Sep 22;12(9):903.

 RVG29-Functionalized Lipid Nanoparticles for Quercetin Brain Delivery and Alzheimer’s Disease.Pinheiro RGR, Granja A, Loureiro JA, Pereira MC, Pinheiro M, Neves AR, Reis S. Pharm Res. 2020 Jul 13;37(7):139.

Double Optimization of Rivastigmine-Loaded Nanostructured Lipid Carriers (NLC) for Nose-to-Brain Delivery Using the Quality by Design (QbD) Approach: Formulation Variables and Instrumental Parameters. Cunha S, Costa CP, Loureiro JA, Alves J, Peixoto AF, Forbes B, Sousa Lobo JM, Silva AC. Pharmaceutics. 2020 Jun 28;12(7):599.

Molecular interactions between Vitamin B12 and membrane models: A biophysical study for new insights into the bioavailability of Vitamin.Ramalho MJ, Andrade S, Coelho MAN, Loureiro JA, Pereira MC. Colloids Surf B Biointerfaces. 2020 Oct;194:111187.

Fluorinated Molecules and Nanotechnology: Future ‘Avengers’ against the Alzheimer’s Disease? Dabur M, Loureiro JA, Pereira MC. Int J Mol Sci. 2020 Apr 23;21(8):2989.

Immuno-nanocarriers for brain delivery: limitations from in vitro to preclinical and clinical studies. Loureiro JA, Ramalho MJ, Carmo Pereira MD. Nanomedicine (Lond). 2020 Mar;15(6):543-545.

La Dra. Loureiro nos ha proporcionado estos enlaces en los que encontraréis información complementaria a su charla:

Liposomas: conceptos básicos (parte I)
Liposomas: conceptos básicos (parte II)

Conferencia: células madre y neurobiología

La próxima #conferenciaMBCM tendrá lugar el viernes, 12 de marzo, a las 10:00 h y será impartida por la Dra. Kristine Freude (University of Copenhagen, Dinamarca). El título de la misma es: Advanced stem cell models to understand neurogenerative diseases. La Dra. Freude ha sido invitada por nuestro compañero, el investigador David Baglietto (Dpto. Biología Celular, Genética y Fisiología), quien se encargará de la presentación.

Debido a la situación de emergencia sanitaria por COVID-19, la conferencia tendrá lugar online a través de la plataforma Google Meet. Podréis acceder mediante el siguiente enlace: https://meet.google.com/iqu-qvoh-axp

Adjuntamos algunas publicaciones recientes de la Dra. Freude:

Microglia-Secreted Factors Enhance Dopaminergic Differentiation of Tissue- and iPSC-Derived Human Neural Stem Cells. Schmidt SI, Bogetofte H, Ritter L, Agergaard JB, Hammerich D, Kabiljagic AA, Wlodarczyk A, Lopez SG, Sørensen MD, Jørgensen ML, Okarmus J, Serrano AM, Kristensen BW, Freude K, Owens T, Meyer M. Stem Cell Reports. 2021 Feb 9;16(2):281-294.

Generation of neural progenitor cells from porcine-induced pluripotent stem cells.Machado LS, Pieri NCG, Botigelli RC, de Castro RVG, de Souza AF, Bridi A, Lima MA, Fantinato Neto P, Pessôa LVF, Martins SMMK, De Andrade AFC, Freude KK, Bressan FF.J Tissue Eng Regen Med. 2020 Dec;14(12):1880-1891.

Glutamate-glutamine homeostasis is perturbed in neurons and astrocytes derived from patient iPSC models of frontotemporal dementia.Aldana BI, Zhang Y, Jensen P, Chandrasekaran A, Christensen SK, Nielsen TT, Nielsen JE, Hyttel P, Larsen MR, Waagepetersen HS, Freude KK. Mol Brain. 2020 Sep 14;13(1):125.

Induced pluripotent stem cells throughout the animal kingdom: Availability and applications.Pessôa LVF, Bressan FF, Freude KK. World J Stem Cells. 2019 Aug 26;11(8):491-505.

Modelling the neuropathology of lysosomal storage disorders through disease-specific human induced pluripotent stem cells. Kobolák J, Molnár K, Varga E, Bock I, Jezsó B, Téglási A, Zhou S, Lo Giudice M, Hoogeveen-Westerveld M, Pijnappel WP, Phanthong P, Varga N, Kitiyanant N, Freude K, Nakanishi H, László L, Hyttel P, Dinnyés A. Exp Cell Res. 2019 Jul 15;380(2):216-233

Generation of two isogenic iPSC lines with either a heterozygous or a homozygous E280A mutation in the PSEN1 gene. Frederiksen HR, Holst B, Mau-Holzmann UA, Freude K, Schmid B. Stem Cell Res. 2019 Mar;35:101403

Generation of two iPSC lines with either a heterozygous V717I or a heterozygous KM670/671NL mutation in the APP gene. Frederiksen HR, Holst B, Ramakrishna S, Muddashetty R, Schmid B, Freude K. Stem Cell Res. 2019 Jan;34:101368

Conferencia MBCM

El próximo viernes 5 de marzo, a las 12 h, tendrá lugar la 2ª conferencia de nuestro ciclo 2020-21. En esta ocasión, la Dra. Claudia Durán (Universidad Adolfo Ibáñez, Chile) nos impartirá la conferencia Contribution of Peripheral Tau Aggregates to Alzheimer´s Disease Pathogenesis. La Dr. Durán ha sido invitada por la investigadora Inés Moreno (Dpto. Biología Celular, Genética y Fisiología, UMA). Debido a la situación de emergencia sanitaria por COVID-19, la conferencia tendrá lugar online a través de la plataforma Google Meet. Podréis acceder mediante el siguiente enlace: meet.google.com/vbo-vpzn-gib

Adjuntamos algunas publicaciones recientes de la Dra. Durán:

DEF8 and Autophagy-Associated Genes are Altered in Mild Cognitive Impairment, Probable Alzheimer’s Disease Patients and a Transgenic Model of the Disease. Leyton E, Matus D, Espinoza S, Benitez JM, Cortes BI, Gomez W, Arévalo NB, Murgas P, Manque P, Woehlbier U, Duran-Aniotz C, Hetz C, Behrens MI, SanMartín CD, Nassif M.J Alzheimers Dis. 2021 Feb 19.

Infusion of blood from mice displaying cerebral amyloidosis accelerates amyloid pathology in animal models of Alzheimer’s disease. Morales R, Duran-Aniotz C, Bravo-Alegria J, Estrada LD, Shahnawaz M, Hu PP, Kramm C, Morales-Scheihing D, Urayama A, Soto C. Acta Neuropathol Commun. 2020 Dec 7;8(1):213.

GERO Cohort Protocol, Chile, 2017-2022: Community-based Cohort of Functional Decline in Subjective Cognitive Complaint elderly. Slachevsky A, Zitko P, Martínez-Pernía D, Forno G, Court FA, Lillo P, Villagra R, Duran-Aniotz C, Parrao T, Assar R, Orellana P, Toledo C, Rivera R, Ibañez A, Parra MA, González-Billault C, Amieva H, Thumala D. BMC Geriatr. 2020 Nov 25;20(1):505

Acute Pannexin 1 Blockade Mitigates Early Synaptic Plasticity Defects in a Mouse Model of Alzheimer’s Disease. Flores-Muñoz C, Gómez B, Mery E, Mujica P, Gajardo I, Córdova C, Lopez-Espíndola D, Durán-Aniotz C, Hetz C, Muñoz P, Gonzalez-Jamett AM, Ardiles ÁO. Front Cell Neurosci. 2020 Mar 19;14:46.

Endoplasmic reticulum proteostasis impairment in aging. Martínez G, Duran-Aniotz C, Cabral-Miranda F, Vivar JP, Hetz C. Aging Cell. 2017 Aug;16(4):615-623.

 Glucose Metabolism: A Sweet Relief of Alzheimer’s Disease. Duran-Aniotz C, Hetz C. Curr Biol. 2016 Sep 12;26(17)